Previously, we reported using U87MG astrocytoma cells and primary cultures of rat cerebral cortical neurons that morphine through activation of MORs and Gαi protein signaling decreased levels of endolysosome Fe2+, increased levels of cytosolic Fe2+, and led to increased levels of ferritin heavy chain [35, 36]. This evidence concerns the gene FTH1 and astrocytoma (excluding glioblastoma).