Serum levels of several tumor markers were elevated; alpha-fetoprotein (AFP) (19.6 ng/ml, normal range < 10 ng/ml), carbohydrate antigen 19–9 (40 U/ml, normal range < 37 U/ml) and pro-gastrin-releasing peptide (103.6 pg/ml, normal range < 67 pg/ml). This evidence concerns the gene AFP and neoplasm.