Moreover, activation of the NLRP3 inflammasome triggered by either bacterial infection, flagellin, or flagellin:antigen fusion proteins might be caused by mitochondrial instability, resulting in mitochondrial ROS production (42), or a shift in overall cell metabolism towards glycolysis, resulting in a disruption of the mitochondrial Krebs cycle, which we previously observed in the rFlaA:Betv1-stimulated BMDMs (5). The gene discussed is NLRP3; the disease is bacterial infectious disease.