To further confirm whether this relationship existed in NSCLC tissue between PCSK9 expression with density and distribution of intratumoral CD8+ T cells, we examined tumor cells and CD8+ T cells in PCSK9hi and PCSK9lo NSCLC tissues by multiplexed immunofluorescent staining (Figure 3). The gene discussed is PCSK9; the disease is non-small cell lung carcinoma.