Using the KEGG enrichment analysis to determine the signaling pathways that involve the DEmRNAs, specific enrichment of genes was observed for signaling pathways including: regulating pluripotency of stem cells, MAPK signaling pathway, calcium signaling pathway, endocrine resistance, PI3k-Akt signaling pathway, protein digestion and absorption, hippo signaling pathway, melanogenesis, pathways of neurodegeneration-multiple diseases, Alzheimer’s disease (Fig. 10A). The gene discussed is AKT1; the disease is early-onset autosomal dominant Alzheimer disease.