In the EL fraction, 1 protein (HBB) was up-regulated and 1 protein (IGHG4) was down-regulated in non-metastatic CRC, while 3 proteins (HP, AGP1, and A1AT) were up-regulated and 1 protein (IGHG4) was down-regulated in the metastatic CRC group, respectively (Fig. 1E and 1F). Here, HP is linked to colorectal carcinoma.