Although other interactions that have been reported associated with Treg development and functions, including TIGIT26, CD22627, 4-1BB28 and LGALS929, were identified, they did not appear to be upregulated in both CD4+ naïve T cells and Treg subtypes, nor significantly strengthed in the eTregs in the NPC microenvironment (Supplementary Fig. 2a–d). Here, CD4 is linked to nasopharyngeal carcinoma.