To further explore the role of the RP11-620J15.3/miR-326/GPI axis in tumor growth in vivo, we subcutaneously injected HCC-LM3 cell lines transfected with miR-326 inhibitors or plasmids containing GPI mRNA that stably knocked out RP11-620J15.3 into BALB/c nude mice. The gene discussed is GPI; the disease is hepatocellular carcinoma.