To determine whether the RP11-620J15.3/miR-326/GPI axis is the key pathway regulating HCC cell proliferation, transformation, and glycolytic activity, we transfected miR-326 inhibitors or plasmids containing GPI mRNA into Huh-7/LM3 cells with RP11-620J15.3 knockdown. This evidence concerns the gene GPI and hepatocellular carcinoma.