We selected significantly deregulated genes from each cell line by comparing the three independent clones to their parental cell line (Supplementary Fig. S5A) and identified 12 upregulated and 13 downregulated genes that were common to all four RAF1-deficient CRC cell lines, defining a RAF1-specific signature (Supplementary Fig. S5B). This evidence concerns the gene RAF1 and colorectal carcinoma.