Genes within hub CHA regulatory units were enriched in immediate early response genes (fold-enrichment: 8.7, P=9.07E − 07, hypergeometric test), in genes involved in cancer (COSMIC database) (fold-enrichment: 3.6, P=0.0001, hypergeometric test) and encoding for transcription factors as reported in the TF checkpoint database (fold-enrichment: 2.1, P=0.01, hypergeometric test) (Fig. 4a). This evidence concerns the gene TF and cancer.