To further uncover the biological effects of RPL32 in HCC cells, we detected the mRNA and protein levels of RPL32 in different HCC cell lines and found that, except for Hep G2, PLC/PRF/5, and SNU‐182 cell lines, the expression level of RPL32 in most HCC cell lines (HCC‐LM3, Huh‐7, MHCC97‐H, SMMC‐7721, and SK‐HEP‐1) was significantly higher than that in normal liver cells (L‐02) (Figure 6A, B). This evidence concerns the gene RPL32 and hepatocellular carcinoma.