First, we thought to determine association of AR-FL and AR-V expression in vitro by using cell lines displaying different AR-FL and AR-V levels as well as diverse stages of prostate cancer (Fig. 1B, S1): RWPE-1 cells (healthy prostate epithelium; AR-FL−/AR-V−), LNCaP (hormone-sensitive stage; AR-FL++/AR-V+), 22Rv1 (castration-resistant stage; AR-FL++/AR-V++), PC-3 (neuroendocrine differentiated stage; AR-FL−/AR-V−) as well as non-prostate and non-cancer, yet AR positive HEK293-T (AR-FL+/AR-V−/+) cells. This evidence concerns the gene AR and Familial prostate cancer.