We performed Hi-C and systematically integrated it with previously published and new ATAC-seq, RNA-seq, and ChIP-seq data from three B-ALL cell lines heterozygous for NSD2 EK (RS4;11, RCH-ACV, SEM) either expressing a NSD2 targeting shRNA or a non-targeting shRNA henceforth referred to as NSD2 Low and High cell lines [5] (Fig 1a). This evidence concerns the gene NSD2 and precursor B-cell acute lymphoblastic leukemia.