CCL5 and viral infectious disease: While we cannot exclude the possibility of off-target effects produced by clodronate and anti-Ly6C mAb on peripheral NK and T cells, resulting in their reduced infiltration into the CNS, the scRNA-seq data shows expression of relevant chemotactic genes by microglia and MCs, including Ccl5, Cxcl9, Cxcl10 and Cxcl16. These are involved in immune cell recruitment in CNS virus infection, making it likely that recruitment to the CNS is largely driven by microglia and MCs [26, 38, 52, 63, 82, 108, 110].