MUC5B and infection: Indeed, using an alternate trajectory analysis tool called Slingshot [80] just on microglial clusters demonstrated two “lineages”, (1) MG1, MG2, MG3, MG4 and MG5 and (2) MG1, MG2, MG3 and MG6, suggesting that upon infection microglia may adopt (1) functionally adaptive, i.e., anti-viral response (MG4) and then immune cell recruiting (MG5) or (2) stressed/dying response, differentially expressing mitochondrial genes (MG3 and MG6) with time (Additional file 3).