Furthermore, wound healing and transwell assays showed that the migration and invasive abilities of MKN45 and AGS cells were enhanced after infection with H. pylori strain (26695 or SS1) infection; however, GNB4 knockdown diminished these abilities (P < 0.001, Fig. 3J–N, Additional file 4: Fig. S4H-L). This evidence concerns the gene GNB4 and infection.