For this study, we primarily focused on genes that are either identified as differentially spliced using both DEXSeq and ISAR tools and reported in at-least one human AD splicing studies (Ppp1r9b, Celf4, and Fars2) or reported in multiple human AD splicing studies and exhibit either differentially used exons or differential transcript usages (MACF1, PPP3CA, ENO2, KIF21A, EFTUD2, STXBP1, PFN2, and PSMD2) (Table 7). The gene discussed is MACF1; the disease is Alzheimer disease.