In the large two-tumor A20 model, the addition of anti-CTLA-4 to CpG + OX40 caused significant local tumor regression compared to PBS and anti-CTLA-4 alone and caused regression of some tumors at the distant untreated site (LCR 5 of 10 at the treated tumor and 4 of 10 at the distant tumor) (Fig. 6c, d, f, g). The gene discussed is TNFRSF4; the disease is neoplasm.