Here, we have expanded urine BMP analysis to other PD-associated mutations that significantly hyperactivate the LRRK2 kinase with greater effect than LRRK2 G2019S such as R1441 hotspot mutations in LRRK2 and the VPS35 D620N mutation that activates LRRK2 kinase activity by a yet unknown mechanism. This evidence concerns the gene VPS35 and Parkinson disease.