Furthermore, mice were infected with GFAP-AAVs to express CAR1 in astrocytes in vDG and then subjected to CSDS (Fig. 5J-M), while CAR1 knockdown via GFAP-AAVs, showed no significant differences in depressive like behaviors, but spent less time in center (Open field test) comparing to GFAP-control group (Fig. S11), this might be related to anxiety like phenotypesOverexpression of CAR1 but not CAR2 (Fig. S12) significantly reduced immobile time in FST comparing to the control group (Fig. 5M). Here, CA2 is linked to Anxiety.