by using TIMER 2.0 online tool and adjusting tumor purity, we found that SPTBN1 expression had negative correlations with the expression levels of TNFSF9 (R =-0.238, p = 2.31E-07), PDCD1 (R =-0.191, p = 3.81E-05) and CTLA4 (R =-0.16, p = 5.67E-04) in KIRC (revised_Figure 6B). This evidence concerns the gene PDCD1 and neoplasm.