F4/80 was a surface‐specific marker of macrophage, CD44 is positively correlated with grade of malignancy and is negatively related to prognosis in various types of tumors, CD44+ TAMCs are in M2 phenotype, suggesting the frequency of M2 phenotype is higher in HCC group than in 3‐HCC group (Figure 3E). This evidence concerns the gene CD44 and hepatocellular carcinoma.