The CyTOF results revealed that the proportion of CD45+F4/80+CD64+CD39+CD44+CD11b+CD11c+PD‐L1+ macrophages was significantly increased, while the proportion of CD45+F4/80−CD64+CD39+CD44+CD11b+CD11c+ macrophages was significantly decreased after 3‐HAA treatment in the mice model of HCC. Here, CD44 is linked to hepatocellular carcinoma.