The expression of genes in module 5 was highest in CD4+ naïve T cells and contained similar gene set enrichments to module 5 in CD8+ MTC (Supplemental Fig. 3i), including self-renewal related transcription factors, such as LEF1. Module 2 was expressed in both CD4+ TCM and TEM subsets as in the CD8+ lineage. This evidence concerns the gene LEF1 and medullary thyroid gland carcinoma.