Notably, we did not detect any changes in expression of necroptosis genes Ripk1 and Ripk3 at 2 or 4 weeks off Dox in rNLS8 mice, despite the detected alterations of apoptosis signalling even at these early timepoints, aligning with recent work suggesting that necroptosis is not a primary driver of neurodegeneration in ALS and FTD [55]. This evidence concerns the gene RIPK3 and amyotrophic lateral sclerosis.