Inference of ligand-receptor pairs from scRNA-seq data suggest that neutrophils release TNFα, which in a paracrine fashion induces expression of MES signature genes in WT-GSC cultures, increases tumor necrosis and a hypoxic response in vivo, in addition to inducing expression of neutrophil recruitment chemokines by tumor cells to further facilitate their infiltration (Fig. 5). The gene discussed is TNF; the disease is neoplasm.