To further confirm that the decreased recruitment of Ly6cHi monocytes leads to increased neutrophils as a result of the effect of qMCPs on the inflammatory monocyte CCR2 receptor, and that the loss of qMCPs does not affect Mg, we orthotopically transplanted freshly dissociated PDGFB-driven GBM cells into Cx3Cr1GFP/WT;Ccr2RFP/WT double heterozygous reporter knock-in and Cx3Cr1GFP/WT;Ccr2RFP/RFP (heterozygous knock-in for Cxc3Cr1 gene and homozygous knock-in for Ccr2) mice. The gene discussed is PDGFB; the disease is glioblastoma.