Conversely, immunostaining against Iba1, revealed that microglia cell density was increased in ALS patients’ retina compared to age matched controls (ALS: 3.3 ± 0.2; n = 35/6 fields/patients; CTRL: 2.4 ± 0.2, n = 36/6 fields/controls; p < 0.01; Figures 4C–E). Here, AIF1 is linked to amyotrophic lateral sclerosis.