To explore whether the elevated PD‐L1 could be verified in vitro, primary human peripheral blood mononuclear cell‐derived Mφ (PBMC Mφ) were co‐cultured with human ovarian cancer cell lines, SKOV3 and OVCAR8, in the presence or absence of IL‐1 receptor antagonist (IL‐1RA). The gene discussed is CD274; the disease is ovarian cancer.