Grey60 module with hub genes such as FDFT1, NSDHL, IDI1, SQLE, ACSS2, SREBF2, HMGCR, FASN, LSS, ACAT2, FADS1, FADS2 and ELOVL6 was upregulated in NASH (Figure 4C), which were mainly participating in cholesterol and lipid metabolic process (Figure 4C). The gene discussed is ACSS2; the disease is metabolic dysfunction-associated steatohepatitis.