For further analysis the expression of the hub genes and key TFs in vitro models of NASH, we retrieved public available RNA-seq data (the RNA-seq data of L02 hepatocytes (PRJNA726826) and murine primary hepatocytes (PRJNA726846) treated with palmitic acid and oleic acid (PAOA) for 0h, 12h and 24h, respectively (42)), we found hub genes (CD53 and SRGN) and key TFs (NFKB1, ELF1 and EST1) displayed higher expression in L02 hepatocytes treated with PAOA (Figure S4A). This evidence concerns the gene SRGN and metabolic dysfunction-associated steatohepatitis.