The top hub genes overexpression in NASH samples and linking immune-related pathways belonged to CD53, LCP1, LAPTM5, NCKAP1L, C3AR1, FGL2, PLEK, HLA-DRA, FPR3 and SRGN, which also showed positive correlation with histological grade (Figure 7C). The gene discussed is CD53; the disease is metabolic dysfunction-associated steatohepatitis.