In cerebral ischemia rats model, the levels of p-ERK and p-CREB were significantly decreased, the activation of ERK/CREB pathway was significantly inhibited, and the expression of pro-inflammatory cytokine IL-6 and TNF-α was increased in post-stroke depression through sucrose preference test (SPT), forced swimming test (FST), tail suspension test (TST), western blot and Elisa experiments (Yin Q. et al., 2021). This evidence concerns the gene CREB1 and major depressive disorder.