IRF3 and acute disseminated encephalomyelitis: We also observed that PRRSV-ADE infection visibly cut down the transcripts of IRF-3, IRF-7, and NF-κB in PAMs in the early stage of infection (after 12–24 h infection) (Supplementary Figures 1A, B), implying that disruption of these three transcription factors may play a crucial part in PRRSV-ADE infection.