Through sgRNA-guided single-base editing, the C>T mutation of the TERT promoter, -124 upstream of the start codons, was corrected to C. This correction blocked the members of the E26 transcription factor family from binding, suppressing the abnormal activation of the TERT promoter and ultimately inducing senescence and proliferation arrest in glioblastoma [32]. This evidence concerns the gene TERT and glioblastoma.