In addition, various functional assays indicated that the attenuation of malignant tumor phenotype by Hsa_circ_0000566 knockdown was rescued by overexpression of HIF-1α, as indicated by the results of CCK-8, EdU, Transwell, colony formation, and apoptosis assays (Fig. 6H-L). The gene discussed is HIF1A; the disease is neoplasm.