GSEA analysis indicated that axon guidance, focal adhesion, pathways in cancer, regulation of actin cytoskeleton, WNT signaling pathway were activated in C1 (Figure 4A), Cytokine-cytokine receptor interaction, MAPK signaling pathway, Neuroactive ligand receptor interaction, pathway in cancer were inhibited in C2 (Figure 4B), Axon guidance, ECM receptor interaction, pathway in cancer and WNT signaling pathway were inhibited in C3 (Figure 4C). The gene discussed is C3; the disease is cancer.