The mRNA expression levels of these genes were verified in human primary bone metastases tumor tissues and normal bone tissues by q-PCR, and the results indicated that the expression levels of PECAM1 decreased and ITGB3 increased significantly, but COL3A1 and CD4 had no significant difference in the expression of bone metastases tissues (Figure 3E). This evidence concerns the gene ITGB3 and neoplasm.