We conducted a systematic search in PubMed, Embase, and the Cochrane Library to identify studies published between January 1, 2010, and January 31, 2023, using the following terms: "stroke," "alteplase," "doses," "efficacy," "tissue plasminogen activator," "r-tPA," and "safety." To identify relevant articles for this study, a comprehensive search was conducted using Medical Subject Headings (MeSH) terms and boolean algebra operators to combine and refine search keywords in a systematic and structured manner. This evidence concerns the gene PLAT and Stroke.