Hyperpolarized pyruvate used to study glucose and glutamine changes induced by DCA in an IDHwt versus an IDHmut glioma cell line.Pyruvate Dehydrogenase (PDH) had a 4-fold lower activity in the IDHmut cell line with concurrent overexpression of PDK mRNA.DCA reactivated PDH more than 700% in in in IDHmut, but only 41% in IDHwt cell lines with 2.4-5.9-fold increase in glutamate levels in IDHmut but only marginal increases in IDHwt.DCA reduced the colony forming effect and proliferation of IDH mutant cell lines. Here, IDH1 is linked to central nervous system cancer.