PDP1 and glioma: Hyperpolarized pyruvate used to study glucose and glutamine changes induced by DCA in an IDHwt versus an IDHmut glioma cell line.Pyruvate Dehydrogenase (PDH) had a 4-fold lower activity in the IDHmut cell line with concurrent overexpression of PDK mRNA.DCA reactivated PDH more than 700% in in in IDHmut, but only 41% in IDHwt cell lines with 2.4-5.9-fold increase in glutamate levels in IDHmut but only marginal increases in IDHwt.DCA reduced the colony forming effect and proliferation of IDH mutant cell lines.