The most enriched pathways of DEGs, including iron ion transport (GO: 0006826) and iron ion homeostasis (GO:0055072) pathways, as determined by Gene Ontology (GO) analysis, are shown in Figure 4B qPCR results further verified that kumatakenin significantly increased enolase (Eno3) expression in colitis mice (Figure 4C). This evidence concerns the gene ENO3 and colitis.