Sun N et al. developed and optimized an HCC-specific EV surface protein detection method (referred as HCC EV ECG score), based on three HCC EV subpopulations of EpCAM+CD63+, CD147+CD63+, and GPC3+ CD63+, by which EVs were isolated from peripheral blood, and HCC EV surface proteins were detected. Here, GPC3 is linked to hepatocellular carcinoma.