PKM and neoplasm: In Colony formation assay (Figures 9A–D and Supplementary Figures S1A–D), Transwell assay (Figures 9E–H and Supplementary Figures S1E–H) and EdU assay (Figures 9I–L and Supplementary Figures S1I–L), compared with the stably knockdown PKM2 cells, PPT1 could significantly promote the proliferation, migration and invasion of HCC tumor cells in stable overexpression of PKM2 between Huh-7 and JHH-7 cells, interestingly, the proliferation, migration and invasion of tumor cells decreased strikingly after the treatment of chloroquine.