The GO and KEGG pathway enrichment showed that DEGs were associated with malignant progression of glioma cells, including nuclear division, ECM component, PDZ domain binding, cell cycle, DNA replication, PI3K-Akt and FoxO signaling pathways, which was partially consistent with the results of bioinformatics analysis in the public database (Figure 8D). The gene discussed is AKT1; the disease is glioma.