High throughput imaging analysis of circulating rare cells (CRCs) purified by WBC subtraction categorized cells into 71 subtypes based on the CD45 leukocyte staining, cell size, chromosome 8 ploidy and the presence of a few tumor cell markers including PD-L1 (EPCAM/CK18/PD-L1/AFP/HER2/CA19-9), endothelial CD31, mesenchymal Vimentin and stem cell CD133 markers (69). The gene discussed is CD274; the disease is neoplasm.