NGB and neoplasm: Western blot analysis showed that activation factors of the tumor angiogenesis pathway were downregulated following NGB-OE in HCT116 and Caco2 cells, including VEGFR2, p-VEGFR2 (Tyr1175), p-Src (Tyr416), p-AKT, and pErk1/2, but not AKT (Fig. 4D).