Among 37 advanced NSCLC patients with EGFR mutation, 23 patients were CYFRA 21 − 1 positive (62.16%; range 3.40 ~ 17.35 ng/mL); 28 patients had high Ki67 (75.68%); 27 patients were CEA positive (72.97%; range 5.610~>1000.00 mg/L); 4 patients were SCC positive (10.81%; range 1.60 ~ 1.74 ng/ml); 13 patients were NSE positive (35.14%; range 16.79 ~ 32.67 ng/mL); 32 patients were TTF-1 positive (86.49%), and CK-7 positive (83.78%). This evidence concerns the gene EGFR and non-small cell lung carcinoma.