Our study showed that patients age ≥ 60 years (p < 0.001), smoking history (p = 0.021), NSE (p = 0.007), ECOG scores (p = 0.012), and TTF-1 (p = 0.022) had significant differences between CYFRA 21 − 1 positive and negative groups, while no differences were observed in gender, family tumor history, SCC, Tumor stages, and CEA (Table 1). Here, ENO2 is linked to neoplasm.