To generate the parental iPSC lines (i.e., LCSBi011A and LCSBi012A), primary skin fibroblasts were obtained from two PD patients carrying the RHOT1 heterozygous mutations c.1290A > G (Miro1 p.T351A) and c.2067A > G (Miro1 p.T610A), which are located in the second EF-hand (EF2) and in the transmembrane (TMD) protein domain, respectively (Berenguer-Escuder et al., 2019). Here, RHOT1 is linked to Parkinson disease.