Meanwhile, deficiency of eaf1 or eaf2 resulted in a dynamic expression of canonical WNT/β-catenin signaling during erythropoiesis, with reduced β-Catenin level and enrichment of the WNT transcriptional factor TCF4 in both scl and lmo2 promoters.This study not only has general implications in regeneration medicine of anemia and related diseases, but also provides evidence that genes eaf1 and eaf2 are important molecules in modulating fish economic or productive traits, such as growth, disease resistance, hypoxia tolerance, and so on. The gene discussed is TCF4; the disease is anemia (phenotype).