We knocked down BUB1B with small-interfering RNA (siRNA) in a TP53-altered (THP-1) and TP53-wild-type (MOLM-13) AML cell line of the same morphological (monocytic) and genetic (KMT2A::MLLT3) subtype to investigate the relative dependency of the mitotic kinase in the growth/survival of these cells. This evidence concerns the gene BUB1B and acute myeloid leukemia.