Interrogation of the DepMap RNAi and CRISPR datasets revealed BUB1B, which encodes a mitotic checkpoint kinase governing proper chromosome separation during cell division, as the upregulated gene whose suppression resulted in significantly lower gene effect scores in TP53-altered than TP53-wild-type AML cell lines (median −1.01 vs. −0.77, P = 0.02) (Fig. 6b and Supplementary Data 4), implicating BUB1B as a more essential gene in AML cells carrying TP53 alterations. The gene discussed is BUB1B; the disease is acute myeloid leukemia.