However, at 60 and 90 min post-infection with M. tb, Nec-1 treatment resulted in a 14.99 ± 8.03% (N = 5) and 28.47±12.64% (N = 5) decrease in pMLKL, respectively, compared to M. tb infected/CREB inhibited macrophages (maroon versus blue bars), reaching significance at 90 min. Here, PCSK1 is linked to infection.