TARDBP and amyotrophic lateral sclerosis: Since the phase separation studies indicated increased aggregation propensity of TDP-43p.Trp385IlefsTer10, we were surprised to see that TDP-43p.Trp385IlefsTer10 exhibited strongly reduced toxic gain-of-function effects compared to WT TDP-43 and ALS-mutant TDP-43 in Drosophila survival and motor performance assays using different drivers.