TARDBP and amyotrophic lateral sclerosis: TDP-43p.Trp385IlefsTer10 has a significantly reduced Csat value compared to WT TDP-43 and TDP-43p.M337V, indicating that TDP-43p.Trp385IlefsTer10 is more prone to phase separation than either the wild-type or the ALS-associated mutant protein (Fig. 4e).