Nan et al. (2022) recently mapped out the numerous Gitelman syndrome-related mutations onto their NCC cryo-EM (single-particle Cryogenic Electron Microscopy) structure and classified them in five groups according to their function or location. In Figure 2C, the mutations appear as various colored spheres: located extracellularly (yellow); in the ion translocation pathway (pink); other TM domains (cyan); TM-C-terminal interface (red); and in the N-terminal domain (green). This evidence concerns the gene SLC12A3 and Gitelman syndrome.