CD4 and tuberculosis: While this study was not specifically designed to assess the performance of biosignature due to the limited number of participants included, we explored this aspect, wherein we assessed the ability of HLA-DR expression on Mtb-specific CD4 T cells to distinguish LTBI from PTB, PCTB, or any active TB (PTB + PCTB) and compared it with previously described biosignatures that included analytes measured in this study.