RNASEH2B and Aicardi-Goutieres syndrome: The D-loop methylation level was significantly higher in AGS patients compared to healthy controls (Figure 1C) as well (-1.747 ± 0.7909; p=0.0168; 95% CI=-3.351 to -0.1429), and the difference was driven by two RNASEH2B-mutated patients carrying the same p.A177T mutation and the IFIH1-mutated patient (Figure 1D).